Atcc hl-60

HL-60 is a promyelocytic cell line derived by S.J. Collins, et al. Peripheral blood leukocytes were obtained by leukopheresis from a 36-year-old Caucasian female with acute promyelocytic leukemia. To ATCC Valued Customers,

Values are means and SD of three independent experiments ( *** p Transfection of HL-60 Cells Use this procedure to transfect plasmid DNA into HL-60 cells in a 24-well format (for other formats, see Scaling Up or Down Transfections, below). All amounts and volumes are given on a per well basis. 1. The day of transfection, count the cells to determine culture density. Plate 1 x 105 cells per well in 0.5 ml of HL-60 CELL LINE RC2DVQ0F6O Overview Names 3: Identifiers 2: Relationships 2: Characteristic Attributes 3: Notes 2: Audit Info References 3: Substance Class Jan 17, 1989 · Although intact ATCC HL-60 cells specifically and saturably take up 1,25-dihydroxy[3H]vitamin D-3, whole cell extracts have little or no specific binding of 1,25-dihydroxyvitamin D-3. The absence of specific binding can now be explained by the action of a serine proteinase in these cells.

10.01.2021

A computational study of the open and closed forms of the N-lobe human serum transferrin apoprotein.. PubMed. Rinaldo, David; Field, Martin J. 2003-12-01. Human serum transferrin tightly binds ferric ions in the blood stream but is able to release them in cells by a process involving receptor-mediated endocytosis and decrease in pH. Oct 30, 2017 · We constructed an effective model of ATRA-induced HL-60 differentiation which described signaling and gene expression events following the addition of ATRA (Fig.

I am dealing with the same issue. I got a HL-60 cells from ATCC and cultured them in IMEM high glucose+20% FBS ( non heat inactivated as recommend by atcc

ZERO BIAS - scores, article reviews, protocol conditions and more Aliases: HL-60. Disease ATCC Gender Female HL60 sequenced and profiled in following datasets. Loss-of-function screens Combined RNAi (Broad, Novartis, Marcotte) The human acute promyelocytic leukemia HL-60 (ATCC® CCL-240™), Jurkat (human acute T lymphocytic leukemia, ATCC® TIB-152™) and K-562 (human chronic myelogenous leukemia, ATCC® CCL-243™) suspension cells were maintained in RPMI-1640, while HepG2 (human hepatocellular carcinoma, ATCC® HB-8065™), MCF7 (human breast adenocarcinoma, ATCC model – HL-60 Study example Human HL-60 cells (ATCC-No: CCL-240) were isolated from a patient with AML (acute myeloid leukemia) and express FLT3 wild type.

I attempted working with HL-60's, but had a low population that consistently differentiated using 1-1.5% DMSO. I switched to NB4 cells to look at chemotaxis, ROS, F-Actin polymerization, etc.

Dorschner Ordering Information HL-60 may be ordered from ATCC as a frozen ampoule.

We optimized the NCI60 cell line panel for generating 3D spheroids of a prespecified diameter (300–500 µm) in ultra-low 20 A549/ATCC. 7500 33 HL -60. 40,000.

HL-60 and KG1 cells were treated for 16 h with 5 µM DDA or 0.1 µM Ara-C, alone or in combination. (A) The presence of acidic vesicles was revealed using Cyto-ID. Data were represented as the % of Cyto-ID-positive cells, in Annexin-V-/7AAD- cells, relative to control cells. ATCC stands ready to support our customers’ needs during the coronavirus pandemic.

HL-60-Luc2 (ATCC® CCL-240-LUC2™). This luciferase expressing cell line was derived from parental line CCL-240 by transduction with lentiviral vector The HL-60 cell line is a human leukemia cell line that has been used for laboratory research on blood cell formation and physiology. HL-60 proliferates Part of: MYC genetic alteration cell panel (ATCC TCP-1035). Registration: National Institute of Standards and Technology, Standard Reference Materials; SRM I am dealing with the same issue. I got a HL-60 cells from ATCC and cultured them in IMEM high glucose+20% FBS ( non heat inactivated as recommend by atcc The HL-60 cell line was derived from peripheral blood leukocytes of a Mixed culture of HL-60 cells (ATCC CCL-240) 96 h after treatment with 0.8% DMF, AND METHODS. Cell Culture. The human promyelocytic leukemia cell line, HL- 60.

hl-60-luc2 atcc ® ccl-240-luc2 ™ frozen 1.0 mL For-Profit: $1,310.00 Non-Profit: $825.30 Article Snippet: HL-60 cells, a promyelocytic leukemic cell line, was acquired from the American Type Culture Collection (ATCC# CCL240) and was maintained in 10% RPMI media with 1% Pen/Strep [ ]. Techniques: HL-60 cells (white arrow) and MDA-MB-231 cells (red arrow) passing through the magnetophoretic separation module under various focusing buffer flow rates and magnetic field strength conditions of (c) 3 mL/h and 0 mT, (d) 3 mL/h and 20 mT, and (e) 5 mL/h and 20 mT. (f – i) Enlarged images of cells during separation from Figure 2 e. The HL-60 cell line is a human leukemia cell line that has been used for laboratory research on blood cell formation and physiology. HL-60 proliferates continuously in suspension culture in nutrient and antibiotic chemicals.

ATCC HL-60, Promyelocytic Leukemia, Frozen Manufacturer: ATCC CCL240 HL-60 cells spontaneously differentiate and differentiation can be stimulated by butyrate, hypoxanthine, phorbol myristic acid (PMA, TPA), dimethylsulfoxide (DMSO, 1% to 1.5%), actinomycin D, and retinoic acid. Nov 29, 2014 · Strategies Cell culture and solutions HL 60 human myeloblastic leukemia cells derived through the original patient isolate, a generous gift of Dr. Robert Gallagher, were grown in RPMI 1640 supplemented with 5% fetal bovine serum and 1x antibiotic antimycotic in the 5% CO2 humidified environment at 37 C. The protocols I have read seem straightforward on differentiating HL-60 cells, however I am not having luck using 1.3% DMSO and/or 1uM ATRA (all trans retinoic acid). I culture in 1640 RPMI The human acute promyelocytic leukemia HL-60 (ATCC® CCL-240™), Jurkat (human acute T lymphocytic leukemia, ATCC® TIB-152™) and K-562 (human chronic myeloge-nous leukemia, ATCC® CCL-243™) suspension cells were maintained in RPMI-1640, while HepG2 (human hepato-cellular carcinoma, ATCC® HB-8065™), MCF7 (human May 22, 2018 · The human promyelocytic leukemia cell line HL-60 (ATCC® CCL240™) was purchased from the American Type Culture Collection (ATCC, USA). HL-60 cells were commonly cultured in RPMI 1640 medium supplemented with 10% ( v /v) fetal bovine serum containing 100 U/ml penicillin and 100 U/ml streptomycin at 37 °C in a humidified atmosphere with 5% CO 2 . The classic human acute promyelocytic leukemia cell line HL-60 was obtained from American Type Culture Collection (ATCC) and maintained in RPMI1640 containing 10% heat-inactivated FBS, 2 μM L-glutamine, 100 U/ml penicillin, and 100 μg/ml streptomycin (Gibco, USA) in a humidified 5% CO 2 atmosphere at 37°C. A computational study of the open and closed forms of the N-lobe human serum transferrin apoprotein..

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Intended uses of ATCC Certified Reference Materials are to: •. Challenge assay CCL-240™ HL-60, human periferal blood myeoblastic cell line vial. 30-2003.

The ATCC Genome Portal makes it easy to find the high-quality whole-genome sequencing data needed for your research. With a free account, you can easily search, download, and analyze hundreds of high-quality genomes. HL-60 from ATCC Description HL-60 is a promyelocytic cell line derived by S.J. Collins, et al. Peripheral blood leukocytes were obtained by leukopheresis from a 36-year-old Caucasian female with acute promyelocytic leukemia.